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Sx 45. Lm 30 ( !. Stocks of EPEC O127H6 strain 2348/69 and S choleraesuis subsp choleraesuis serotype Typhimurium (S Typhimurium, DSMZ 5569) were maintained frozen at )70 C until use Overnight cultures were grown at 37 C on agar plates using Columbia agar base with 10% (w/v) defibrinated horse blood (Oxoid, Basingstoke, Hampshire, UK).

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